Nanotech 2013 Vol. 1
Nanotech 2013 Vol. 1
Nanotechnology 2013: Advanced Materials, CNTs, Particles, Films and Composites (Volume 1)

Nanostructured Materials & Devices Chapter 10

Background free biosensor using the combination of graphene oxide and Fe3O4 nanoparticles

Authors: S. Cho, L. Park, R. Chung, J.H. Lee

Affilation: Luminescent MD, LLC, United States

Pages: 711 - 712

Keywords: biosensor, graphene oxide, chemiluminescence, aptamer, magnetic nanoparticles

Abstract:
Cost-effective and rapid biosensor with background free chemiluminescence (CL) detection was developed using ssDNA aptamer-conjugated 6-FAM fluorescein (DAF), graphene oxide bound with magnetic nanoparticle (GMN), and 3,4,5-trimethoxyphenylglyoxal (TMPG). When thrombin was incubated in TE buffer (pH 7.5) containing 200 nM DAF for 10 minutes, thrombin binds strongly to DAF. Free DMF, which does not bind with thrombin in the solution, binds with GMN through the - interaction between the DAF and GMN. Thus, we were able to remove DMF-bound GMN in the solution using a magnetic bar. When thrombin-bound DMF was mixed with TMPG (0.1 M) in the presence of tetrapropylammonium hydroxide (0.01 M), strong CL was observed based on the chemiluminescence resonance energy transfer from high-energy intermediate formed from the reaction of TMPG and guanine, the main base of DAF, to 6-FAM. The background-free biosensor having excellent accuracy and precision was more sensitive than conventional and time-consuming analytical methods.


ISBN: 978-1-4822-0581-7
Pages: 830
Hardcopy: $209.95