Chen E.T., Shen J. Thornton Y., Ngatchou C., Duh S-H, Kissinger P.T.
Advanced Biomimetic Sensors, Inc., US
Keywords: contour map multiple variable correlation method, heat release, nanopore biomimetic membrane, quantitatively detects early stage cancer cells, ratio of action potential, resting potential
Detecting low abundance cancer cell is challenged by the complex instrumentation and time consuming assays. We report a unique cancer cell detection device that is capable of detecting low abundance MDA-MB-231 breast cancer cells based on a nanopore biomimetic cross linked polymer membrane fabricated on gold chips. The breast cancer cells were detected by changes of an action potential when receptor of the cancer cell directly interacts with the active site of the Au/membrane. This change of action potential is recorded using a double step chronopotentiometry (DSCPO) method under labeling-free and antibody-free conditions. Results were compared with a normal breast tissue that did not have interaction. This study was built upon our previous work using nanopore sensor to measure glucose:(Nanotech (3),101-104, 2011). The results show a significant difference of released heats between 5 cancerous cells and a normal breast tissue (0.010 calorie vs. 0.001 calorie). The difference was positively correlated with the high abnormal ratio of action/resting potential as discharge current rose to mA level. The sensitivity of the device is 320 mV/cell.mL-1 corresponding to 5 cell/mL. Effect of cell concentration (1-200 cell/mL) on action and resting potential profiles is reported.
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Published: June 18, 2012
Pages: 198 - 201
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topics: Biomaterials, Cancer Nanotechnology
ISBN: 978-1-4665-6276-9