 | Nanotech 2012 Vol. 3
Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Chapter 1: Biosensing, Diagnostics & Imaging |
Continuous Sensing of blood by dark-field microscopy and surface-enhanced Raman Spectroscopy | |
| Authors: | A.-I. Henry, B. Sharma, L.B. Sagle, R.P. Van Duyne |
| Affilation: | Northwestern university, US |
| Pages: | 40 - 43 |
| Keywords: | chemical sensing, SERS, dark-field microscopy |
| Abstract: | Surface-enhanced Raman spectroscopy (SERS) is a rapid, label-free technique with multiplexing capabilities, making it very relevant for the sensitive real-time detection for biomedical applications,, . Demonstration of in vivo glucose detection with unprecedented sensitivity (micromolar to picomolar concentration sensitivity) in a rat model has been made recently in our group. We have developed a combined SERS-active microfluidic substrate that has been integrated with a SERS imaging system for the detection of biomarkers. The SERS substrate has been functionalized with optimized surface chemistries (mixed self-assembled monolayers) for the capture of small molecules and bacteria. Microfluidic channels on top of the SERS-active surface allow for flowing blood, which is distributed over the substrate (see Figure). SERS imaging uses a line focus parallel to the channels, which accounts for measuring the SERS signal continuously and visualize the SERS activity across the microfluidic channel with the imaging capability of this system. |
| ISBN: | 978-1-4665-6276-9 |
| Pages: | 818 |
| Hardcopy: | $209.95 |
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