New microfluidic chip for the production of spherical gelled capsule for cell encapsulation

	Nanotech 2012 Vol. 2 Nanotechnology 2012: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational (Volume 2) Chapter 5: Micro & Nano Fluidics
	New microfluidic chip for the production of spherical gelled capsule for cell encapsulation
Authors:	P. Dalle, S. Morales, J. Berthier, P-Y. Benhamou, A. Bellemin Comte, F. Boizot, G. Costa, F. Rivera
Affilation:	CEA/LETI, FR
Pages:	298 - 301
Keywords:	microfluidic, alginate, pancreatic islet, encapsulation, automation, phase transfer
Abstract:	Implantation of Langerhans islet in alginate shell is an alternative for the treatment of diabetes type 1. Finding an adapted process for cell encapsulation is a challenge. The process must product spherical and monodisperse capsules in a limited time with a good reproducibility. Up to now conventional systems are mainly manual which induces batch disparities and do not provide repeatable clinical results. In this paper, we report a novel microfluidic device coupling a production of alginate droplets in an oil flow and a phase transfer module, that automatically transfers the capsules in an aqueous phase. This module allows a 100% extraction of alginate capsule from the oil phase to a calcium gelling solution. Gelled spherical capsules are directly retrieved in an aqueous phase. The entire process is done at physiological pH without any surfactant.
ISBN:	978-1-4665-6275-2
Pages:	878
Hardcopy:	$209.95

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