Nano Science and Technology Institute
Nanotech 2011 Vol. 3
Nanotech 2011 Vol. 3
Nanotechnology 2011: Bio Sensors, Instruments, Medical, Environment and Energy
 
Chapter 5: Cancer Nanotechnology
 

New High Conductance DEP Devices for Detection of Cancer Related DNA Nanoparticulate Biomarkers and Nanoparticles in Whole Blood

Authors:M.J. Heller, A. Sonnenberg
Affilation:University of California San Diego, US
Pages:360 - 363
Keywords:cancer, biomarkers, nanoparticles, DEP, detection, blood
Abstract:The ability to rapidly detect cell free circulating (cfc) DNA nanoparticulate biomarkers, drug delivery nanoparticles and virus directly from blood is a major challenge for nanomedicine. We have now developed unique, simple and practical high conductance dielectrophoretic (HC-DEP) devices which allow cfc-DNA, cellular nanoparticles (mitochondria, etc.), nanoparticles and virus to be rapidly isolated from undiluted whole blood. The new HC-DEP devices are simple capillary tube and pipette devices which can be inserted directly into a few microliters of whole blood. An AC voltage from 50-150 volts is applied at frequencies of 5kHz-10kHz which causes cfc-DNA (nanoparticles, virus) to become concentrated on the tip of the device. The material captured on the tip can then be transferred to a collection tube for further analysis (PCR, genotyping, sequencing). The new HC-DEP devices are being used to collect cfc-DNA from Chronic Lymphocytic Leukemia (CLL) whole blood patient samples. Blood sample to PCR is achieved in less than twenty minutes. HC-DEP sets the stage for new “seamless” sample to answer diagnostic systems which will allow a variety of important cancer and other disease biomarkers to be rapidly isolated and analyzed directly from blood and other clinical samples.
New High Conductance DEP Devices for Detection of Cancer Related DNA Nanoparticulate Biomarkers and Nanoparticles in Whole BloodView PDF of paper
ISBN:978-1-4398-7138-6
Pages:852
Hardcopy:$199.95
 
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