 | Nanotech 2011 Vol. 3
Nanotechnology 2011: Bio Sensors, Instruments, Medical, Environment and Energy
Chapter 5: Cancer Nanotechnology |
Polymersomes and Imaging: A Quantitative Approach | |
| Authors: | A.S. Joseph, N.J. Brown, G. Battaglia |
| Affilation: | University of Sheffield, UK |
| Pages: | 371 - 374 |
| Keywords: | Polymersomes, fluorescence, HPLC, imaging |
| Abstract: | Nano-sized carriers are widely used as imaging tools. Quantification of the amount of carrier delivered provides useful informations in development and testing stages. This work describes a HPLC method to quantify fluorescent labelled polymersomes. Polymersomes are nanometer-sized vesicles made by the self-assembly of amphiphilc co- polymers in water . We use pH sensitive PMPC25-PDPA70 and PEO113 -PDPA70 co-polymers labelled with Rhodamine 6G. We analyse interactions of these two formulations with different cell populations using quantitative fluorescence microscopy imaging, fluorescence activated cell sorting (FACS) and HPLC quantification. A method is here developed to quantitatively recover and quantify polymersomes from cell lysate. Cellular studies are performed in static and in flow conditions, using mouse fibrosarcoma cells over-expressing different isoform of VEGF. Epifluorescence analysis showed that overtime there is an increase of up-take for both formulations. FACS analysis confirmed this trend . A significant difference was found between the two formulations in terms of percentage of cells up-taking fluorescent labelled polymersomes. The HPLC method we developed confirmed this difference and it also allowed to measure the quantity of labelled polymers up-taken. These results will provide the basis for the development of vesicles with targeting moieties. |
| ISBN: | 978-1-4398-7138-6 |
| Pages: | 852 |
| Hardcopy: | $199.95 |
| Order: | Mail/Fax Form |
| Special: | 3 CD Set — 15% off with Free Shipping |
| Up |
© 2013 Nano Science and Technology Institute. All Rights Reserved.
Terms of Use | Privacy Policy | Contact Us | Site Map
Terms of Use | Privacy Policy | Contact Us | Site Map