Authors: S.K. McGraw, E.C. Alocilja, K.J. Senecal, G.N. Prata, A.G. Senecal
Affilation: Michigan State University, United States
Pages: 56 - 59
Keywords: electrochemical biosensor, antibody immobilization, electrotextile, food safety, immunosensor
Research is being performed to develop rapid and novel electrochemical biosensors for the detection of food pathogens at the US Army NSRDEC. The focus of the research is fiber based platforms to perform both capture and sensing functions in a single component. The biosensor will be based on the use of nonwoven micro and nanofiber membranes coated with a conductive polymer and functionalized with antibodies for biological capture. The study will look at various methods for the immobilization of antibodies onto the three dimensional conductive fiber surfaces in order to optimize the specific capture and sensing of a target pathogen. Ultimately, the nonwoven membrane platform will be capable of being dipped into a test sample to act as a pathogen collector and then can be inserted into the electrochemical cell to complete the biosensor circuit by also functioning as the working electrode. The results of this study show that biological recognition elements can be attached to conductive polymer coated non-woven fibers through functional groups built into the polymer coating. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.