Nano Science and Technology Institute
Nanotech 2011 Vol. 3
Nanotech 2011 Vol. 3
Nanotechnology 2011: Bio Sensors, Instruments, Medical, Environment and Energy
 
Chapter 1: Bio Sensors, Diagnostics, Imaging & Instrumentation
 

Capacitance-based real time monitoring of endocytosis

Authors:R.M. Lee, P. Kim, K. Kim, K.-M. Kim, D. Kim, C.O. Yun, J.-S. Shin, K.-H. Yoo
Affilation:Yonsei University, KR
Pages:44 - 47
Keywords:capacitance sensor, receptor mediated endocytosis, pinocytosis, phagocytosis
Abstract:There exist three kinds of endocytosis; receptor mediated endocytosis, pinocytosis and phagocytosis. First, receptor-mediated endocytosis is essential for targeted gene/drug delivery to a specific cell type. Second, pinocytosis is the engulfing and digestion of dissolved substances and phagocytosis is the engulfing and digestion of microscopically visible particles. In this study, we developed a capacitance sensor to monitor and distinguish three kinds of endocytosis in real time. The capacitance sensor is fabricated simple photolithography process on glass substrate and target cells are positioned between gap electrodes. The capacitance sensor was able to detect a capacitance peak in different cell lines during the internalization of adenoviruses or antibodies via receptor-mediated endocytosis. In contrast, the capacitance declined without a capacitance peak when nanoparticles were taken up via non-specific pinocytosis. In case of phagocytosis, the capacitance shows a peak during the engulfing of bacterium, and then capacitance is abruptly increases due to the competition between engulfing the bacterium and bacteria dividing. Thus, our capacitance sensor represents a potential capacitance-based means of discrimination among receptor-mediated endocytosis, non-specific pinocytosis and phagosytosis. Moreover, we developed a capacitance sensor array to demonstrate capacitance-based high-throughput screening
ISBN:978-1-4398-7138-6
Pages:852
Hardcopy:$199.95
 
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