Authors: I.L. Hsiao, Y.J. Huang
Affilation: National Tsing Hua University, Taiwan
Pages: 557 - 560
Keywords: nano-ZnO, cytotoxicity, serum, agglomeration state
The aim of this study is to evaluate the influence of serum in cell culture medium on nanotoxicity to human lung epithelial cells (A549). We found nano-ZnO had greater toxicity as it was suspended by serum-free medium, which could be attributed to serious agglomeration,rapid settlement of nanoparticles and starved cells. Nano-ZnO in 5% and 10% serum-contained medium had the same stable suspensions, and yet caused different toxicological responses in MTT and IL-8 assay. Though nanoparticles in serum-contained medium was stable, they still did not approach the real size distribution in air, which was monitored by Electrical Low Pressure Impactor (ELPI). In conclusion, serum-contained medium might be an appropriate environment to assess cytotoxicity of nano-ZnO, while its dispersibility still needed to be optimized and the content of serum should be regulated.