Nano Science and Technology Institute
Nanotech 2010 Vol. 3
Nanotech 2010 Vol. 3
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
 
Chapter 7: Nano Medical Sciences
 

Nanoscale structure and property changes in collagen fibrils on the extracorporeal shockwave therapy applications of the rat Achilles tendon injury

Authors:S. Choi, G.-J. Lee, J. Chon, S.-J. Chae, S.-D. Yoo, J.-H. Park, K.-S. Kim, H.-K. Park
Affilation:Kyung Hee University, KR
Pages:478 - 481
Keywords:extracorporeal shockwave therapy, nanoscale structure, property, Achilles tendons, collagen fibrils, Atomic force microscopy
Abstract:Extracorporeal shockwave therapy (ESWT) is popular in the treatment of musculo-skeletal injuries. However, the mechanism by which such therapy might have an effect on tendon injury is unknown. The Achilles tendons are mainly composed of Type I collagen fibers including collagen fibrils. Therefore, the aim of this study was to observe the effects of ESWT in healing process of the rat collagenase-induced Achilles tendonitis (CIAT) model. Seventeen male Sprague-Dawley rats were used. An induction of CIAT model was elicited by an injection of 30 μl crude collagenase into the bone-tendon junction of both hindpaws. Fifteen CIAT rats were sacrificed properly at 2d-63d after collagenase injection. 7d-33d rats were treated by ESWT. Atomic force microscopy (AFM) imaging and adhesion force was measured using the non-contact and contact mode. Ultrastructure and property changes in the Achilles tendon with and without ESWT application were evaluated by diameter and D-banding of collagen fibrils, and adhesion force. In CIAT rats, fibril diameter and adhesion force showed significant maximum increases (p<0.0001) at 40d after. Interestingly, the collagen fibril diameter and adhesion force in CIAT rats with ESWT application showed the maximum increases (p<0.0001) at 12d after injection. Further, the pattern of change in two parameters during period (7d-26d) with ESWT was similar to that of post-period (33d-63d) without ESWT. We suggest that ESWT might have the effect in reducing the healing period in the Achilles tendonitis.
Nanoscale structure and property changes in collagen fibrils on the extracorporeal shockwave therapy applications of the rat Achilles tendon injuryView PDF of paper
ISBN:978-1-4398-3415-2
Pages:880
Hardcopy:$189.95
 
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