Authors: V.M. Balcão, A.F. Azevedo, L.M. Castro, C.I. Costa, S. Santos, C.M. Matos, C.G. Moutinho, J.A. Teixeira, J.C. Azeredo
Affilation: University Fernando Pessoa and Institute for Biotechnology and Bioengineering, Portugal
Pages: 459 - 462
Keywords: bacteriophage, nanoencapsulation, multiple emulsions
of the optimized bacteriophage-encasing nanovesicles encompassed determination of particle hydrodynamic size, size distribution and particle charge via DLS, surface morphology via CRYO-SEM, and thermal analysis via DSC, whereas antimicrobial activity of the nanoemulsions produced was assessed in vitro using several bacterial strains. The optimized nanosystems showed no phase separation and encompassed nanovesicles with an average size of ca. 114 nm and an average Zeta Potential of ca. -13 mV, which were maintained stable over a storage timeframe of ca. 3 months. The method of inhalational targeting may have a high potential for the treatment of respiratory bacterial infections because the aerosol is delivered directly at the site of infection, thereby accelerating the action of bacterial predators, whereas protection for the bacteriophage against the immune system is provided by the lipid nanovesicles.