Nano Science and Technology Institute
Nanotech 2010 Vol. 3
Nanotech 2010 Vol. 3
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
 
Chapter 5: Drug & Gene Delivery
 

Synthesis and characterization of methoxy poly(ethylene glycol)-O-chitosan-polyethylenimine as a noviral carrier for gene delivery and expression

Authors:Y.Y. Yu, Z. Wang, L. Cai, G. Wang, X. Yang, Z.G. Xu, X.P. Wan, X.H. Xu, Y. Li, Q. Jiang, R. Gao
Affilation:Sichuan University, CN
Pages:334 - 337
Keywords:chitosan modification, nanoparticls, GFP gene, transfection, expression, real time quantitative PCR
Abstract:A novel water-soluble chitosan (CS) derivative,methoxy poly(ethylene glycol)-O-chitosan- polyethylenimine(mPEG-O-CS-PEI), was synthesized by grafting polycationic polyethylenimine (PEI) and methoxy poly (ethylene glycol) (mPEG) onto chitosan, the copolymer was characterized and confirmed by 1H-NMR and FT-IR spectra. The particle size and zeta potential of mPEG-O-CS-PEI/plasmid complexes were respectively 65 nm and +28.5 mV at the mass ratio of 20:1. Agarose gel electrophoresis confirmed that DNA was retained completely by the copolymer nanoparticles, indicating good DNA package of mPEG-O-CS-PEI nanoparticles. The transfection of L-02 cells proved that mPEG-O-CS-PEI/ plasmid nanoparticles only had weak toxicity on the growth of cells compared with that of chitosan. The result of real time quantitative PCR and GFP expression imaging showed that the transfection efficiency of mPEG-O-CS-PEI was significantly higher than PEI and Lipofectin in L-02 cells (P<0.05). Furthermore, the toxicity of mPEG-O-CS-PEI/plasmid nanoparticles was much lower than those of PEI and Lipofectin molecules(P<0.05). Therefore, mPEG-O-CS-PEI copolymer is a safe, effective and promising cationic polymer carrier for nonviral gene therapy of animals in the future.
ISBN:978-1-4398-3415-2
Pages:880
Hardcopy:$189.95
 
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