Nanotech 2010 Vol. 3
Nanotech 2010 Vol. 3
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy

Bio Sensors, Diagnostics & Imaging Chapter 1

Intracellular Analysis by SERRS and Nanoparticles

Authors: R. Stevenson, L. Senior, J. Alexander, M. Geboers, K. Faulds, D. Graham

Affilation: University of Strathclyde, United Kingdom

Pages: 153 - 156

Keywords: SERRS, SERS, nanoparticles, intracellular detection

Abstract:
We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.

Intracellular Analysis by SERRS and Nanoparticles

ISBN: 978-1-4398-3415-2
Pages: 880
Hardcopy: $189.95