Nano Science and Technology Institute
Nanotech 2010 Vol. 3
Nanotech 2010 Vol. 3
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
 
Chapter 1: Bio Sensors, Diagnostics & Imaging
 

Facile Fabrication of an Enzyme Microreactor Using Magnetic Microbeads

Authors:X. Liu, R.C. Lo, F.A. Gomez
Affilation:California State University, Long Beach, US
Pages:141 - 144
Keywords:diaphorase, enzyme, magnetic microbeads, microfluidics, microreactor
Abstract:In this paper, we present a simple method to fabricate an enzyme microreactor using soft lithography and magnetic microbeads. For proof of concept, we performed an enzymatic reaction by flowing non-fluorescent resazurin through diaphorase (DI) (E.C. 1.6.99) covalently attached to magnetic microbeads packed in a PDMS microfluidic chip to convert it to fluorescent resorufin in the presence of nicotinamide adenine dinucleotide phosphate (NADH).
ISBN:978-1-4398-3415-2
Pages:880
Hardcopy:$189.95
 
Order:Mail/Fax Form
Up
© 2014 Nano Science and Technology Institute. All Rights Reserved.
Terms of Use | Privacy Policy | Contact Us | Site Map