Authors: X. Liu, R.C. Lo, F.A. Gomez
Affilation: California State University, Long Beach, United States
Pages: 141 - 144
Keywords: diaphorase, enzyme, magnetic microbeads, microfluidics, microreactor
In this paper, we present a simple method to fabricate an enzyme microreactor using soft lithography and magnetic microbeads. For proof of concept, we performed an enzymatic reaction by flowing non-fluorescent resazurin through diaphorase (DI) (E.C. 1.6.99) covalently attached to magnetic microbeads packed in a PDMS microfluidic chip to convert it to fluorescent resorufin in the presence of nicotinamide adenine dinucleotide phosphate (NADH).