Facile Fabrication of an Enzyme Microreactor Using Magnetic Microbeads

	Nanotech 2010 Vol. 3 Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy Chapter 1: Bio Sensors, Diagnostics & Imaging
	Facile Fabrication of an Enzyme Microreactor Using Magnetic Microbeads
Authors:	X. Liu, R.C. Lo, F.A. Gomez
Affilation:	California State University, Long Beach, US
Pages:	141 - 144
Keywords:	diaphorase, enzyme, magnetic microbeads, microfluidics, microreactor
Abstract:	In this paper, we present a simple method to fabricate an enzyme microreactor using soft lithography and magnetic microbeads. For proof of concept, we performed an enzymatic reaction by flowing non-fluorescent resazurin through diaphorase (DI) (E.C. 1.6.99) covalently attached to magnetic microbeads packed in a PDMS microfluidic chip to convert it to fluorescent resorufin in the presence of nicotinamide adenine dinucleotide phosphate (NADH).
ISBN:	978-1-4398-3415-2
Pages:	880
Hardcopy:	$189.95

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