Nano Science and Technology Institute
Nanotech 2010 Vol. 3
Nanotech 2010 Vol. 3
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
Chapter 1: Bio Sensors, Diagnostics & Imaging

Functionalized Nanopipette: towards single cell immunoassay

Authors:P. Actis, O. Jejelowo, N. Pourmand
Affilation:UC Santa Cruz, US
Pages:88 - 90
Keywords:nanopipette, immunoassay, label-free, biosensor
Abstract:Nanopipette technology is capable of detecting and functional analyzing bio-molecules based on difference on their size, shape and electrical charge. This unique label-free biosensor is inexpensive, easy to fabricate and versatile. Nanopipette functionalized with antibodies specific to HT-2 toxin, a food-borne pathogen, showed a limit of detection for HT-2 toxin as low as 100 fg/ml with a dynamic range of 4 logs. We are currently investigating the application of functionalized nanopipette for HPV proteins detection. A highly sensitive nanopipette probe can be precisely positioned at any sub-cellular region, thus paving the way for single cell immunoassay.
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