Nano Science and Technology Institute
Nanotech 2009 Vol. 2
Nanotech 2009 Vol. 2
Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites
 
Chapter 7: Environment, Water, Health, Safety and Society
 

Enhanced Biodegradation of 2,3,4,5,6-pentachlorocyclohexan-1-ol -- a b-HCH Metabolite -- in the Presence of a and g-HCH Isomers by Pseudomonas aeruginosa ITRC-5

Authors:A. Singh, A. Kumar, K. Misra
Affilation:Rice University, US
Pages:428 - 431
Keywords:pseudomonas aeruginosa ITRC-5, b-Hexachlorocyclohexane, 2; 3; 4; 5; 6 -pentachlorocyclohexan-1-ol, biodegradation
Abstract:Chlorinated insecticide hexachlorocyclohexane (HCH), which is still used in many countries in agriculture and forestry, leads to environmental problems. HCH consists of a mixture of four isomers: a-, b-, g-, and d. Efforts have been made for the remediation of soils and groundwater contaminated with the toxic and persistent HCH isomers through biodegradation processes. A bacterium pseudomonas aeruginosa ITRC-5 degrades not only a-, g-, and d- HCH isomers of but also b-HCH which is the most recalcitrant due to its chemical stability. The degradation of b-isomer is accompanied with the formation of a metabolite 2,3,4,5,6-pentachlorocyclohexan-1-ol (PCCOL). In the present study, effectiveness of ITRC-5 strain in further degradation of PCCOL was evaluated. g-HCH grown ITRC-5 cells show higher degradation of PCCOL than the t-HCH grown cells do. Furthermore, degradation of PCCOL by g-HCH grown cells is enhanced in the presence of a- or g-HCH isomers. Cloned genes of ITRC-5 bacterium showed 50% degradation of PCCOL in 16 hrs. The ITRC-5, therefore, demonstrates potential for the bioremediation of b-HCH and PCCOL.
ISBN:978-1-4398-1783-4
Pages:590
Hardcopy:$179.95
 
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