Nano Science and Technology Institute
Nanotech 2009 Vol. 2
Nanotech 2009 Vol. 2
Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites
Chapter 6: Nano Bio Materials and Tissues

Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis

Authors:W. Wang, Z. Li, D.I.C. Wang
Affilation:National University of Singapore, SG
Pages:337 - 339
Keywords:magnetic nanoparticles, immobilization of enzymes, core-shell, superparamagnetism, biocatalysis
Abstract:In this paper, iron oxide magnetic nanoparticles were synthesized by co-precipitation method and were functionalized with aldehyde group. Spherical superparamagnetic nanoparticles with diameter of 100 nm were obtained based on the results of field emission scanning electron microscopy (FESEM) and vibrating sample magnetometer (VSM). These particles were further applied for immobilization of enzymes by covalent binding between aldehyde group on particles and amino group on the enzyme surface. Different pH was applied for optimization of the immobilization conditions. The result showed that pH 4.0 is the best pH for immobilizing BSA on the nanometric support.
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