Authors: Y-N. Lin, R-Q. Hsu, K-M. Chang
Affilation: National Chiao Tung University, Taiwan
Pages: 749 - 751
Keywords: polylactic acid (PLA), hyaluronic acid (HA), electrospinning
In the study of biomimetic material design, mimicking nanostructured thin film components can take the place of the extracellular matrix. The new types of thin film were fabricated by polylactic acid (PLA) and hyaluronic acid (HA) composite nano-fibers with three different polylactic acid (PLA) to hyaluronic acid (HA) ratios (PLA:HA= 95:5 wt %, 90:10 wt% and 85:15 wt% ). The composite nano-fibers produced by electrospinning has high tensile strength. The materials analysis with nano-fibers was demonstrated via Fourier transform infrared (FT-IR), Thermal gravimetric analysis (TGA) and scanning electron microscope (SEM). In the present study, we assessed the response of mouse feeder cells and NIH-3T3 cells to different ratios of composite nano-fibers with regard to cellular morphology, proliferation, formation of intercellular contacts as well as integrin-depent adhesion. Fibroblast cells attachment and proliferation of composite nano-fibers were discussed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scanning electron microscope (SEM) observation. These results suggested that the composite nano-fibers have good biocompatibility in fibroblast cells and stimulate fibroblast synthesis collagen.