 | Nanotech 2008 Vol. 2
Nanotechnology 2008: Life Sciences, Medicine & Bio Materials - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Chapter 6: Nano Medicine & Neurology |
Nanoparticle-enhanced Magnetic Field Induces Apoptosis in Nematode | |
| Authors: | G.S. Huang, L.K. Yeh, Y.C. Chen |
| Affilation: | National Chiao Tung University, TW |
| Pages: | 497 - 500 |
| Keywords: | apoptosis, nanoparticles, magnetic field, C. elegans |
| Abstract: | To investigate molecular consequence of magnetic field to C. elegans, Fe3O4 nanoparticles were incorporated into the culture media of C. elegans and the nematodes were grown under magnetic field for 4 days. For the expermental group, we observed a 30 to 50 percents reduction in the moving speed. However, only nematode at L4 stage exhibited this difference. To explore molecular mechanisms affected by this treatment, real-time RT-PCR was performed using primer paris specific for 120 genes. Differential expression for genes belonging to apoptosis was observed. Biochemical assay for Caspase 3 activity was performed. The magnetic field-treated nematode exhibited high levels of Caspase 3 activity consistent with real-time PRC. Fluorescence microscopy showed that apoptotic activity was associated with the Fe3O4 treatment. For further validation, a ced-3 mutant nematode under enhanced magnetic field showed less effect for the movement than wild-type. The current study indicated that nanoparticle-enhanced magnetic field induced apoptosis in nematode. |
| ISBN: | 978-1-4200-8504-4 |
| Pages: | 808 |
| Hardcopy: | $159.95 |
| Order: | Mail/Fax Form |
| Special: | 3 CD Set — 15% off with Free Shipping |
| Up |
© 2013 Nano Science and Technology Institute. All Rights Reserved.
Terms of Use | Privacy Policy | Contact Us | Site Map
Terms of Use | Privacy Policy | Contact Us | Site Map