Nanotech 2008 Vol. 2
Nanotech 2008 Vol. 2
Nanotechnology 2008: Life Sciences, Medicine & Bio Materials - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2

Nano Medicine & Neurology Chapter 6

Nanoparticle-enhanced Magnetic Field Induces Apoptosis in Nematode

Authors: G.S. Huang, L.K. Yeh, Y.C. Chen

Affilation: National Chiao Tung University, Taiwan

Pages: 497 - 500

Keywords: apoptosis, nanoparticles, magnetic field, C. elegans

Abstract:
To investigate molecular consequence of magnetic field to C. elegans, Fe3O4 nanoparticles were incorporated into the culture media of C. elegans and the nematodes were grown under magnetic field for 4 days. For the expermental group, we observed a 30 to 50 percents reduction in the moving speed. However, only nematode at L4 stage exhibited this difference. To explore molecular mechanisms affected by this treatment, real-time RT-PCR was performed using primer paris specific for 120 genes. Differential expression for genes belonging to apoptosis was observed. Biochemical assay for Caspase 3 activity was performed. The magnetic field-treated nematode exhibited high levels of Caspase 3 activity consistent with real-time PRC. Fluorescence microscopy showed that apoptotic activity was associated with the Fe3O4 treatment. For further validation, a ced-3 mutant nematode under enhanced magnetic field showed less effect for the movement than wild-type. The current study indicated that nanoparticle-enhanced magnetic field induced apoptosis in nematode.

Nanoparticle-enhanced Magnetic Field Induces Apoptosis in Nematode

ISBN: 978-1-4200-8504-4
Pages: 808
Hardcopy: $159.95