Nano Science and Technology Institute
Nanotech 2008 Vol. 2
Nanotech 2008 Vol. 2
Nanotechnology 2008: Life Sciences, Medicine & Bio Materials - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Chapter 4: Drug & Gene Delivery Systems

Real-time Detection and Quantitation of siRNAs in vivo

Authors:A. Cheng, Y. Liang, A. Vlassov, M. Li, Y. Wang, L. Wong, C. Chen, S. Magdaleno
Affilation:Ambion Inc., An Applied Biosytems Business, US
Pages:427 - 429
Keywords:RNAi, in vivo, siRNAs, detection
Abstract:We have developed TaqMan based RT-PCR assays to detect and quantitate siRNAs in vivo. These assays can be used to measure siRNA delivery efficiency after injections and follow body distribution. These assays can also be used to understand the RNAi mechanism and pathways in vivo.
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