Authors: A. Cheng, Y. Liang, A. Vlassov, M. Li, Y. Wang, L. Wong, C. Chen, S. Magdaleno
Affilation: Ambion Inc., An Applied Biosytems Business, United States
Pages: 427 - 429
Keywords: RNAi, in vivo, siRNAs, detection
We have developed TaqMan based RT-PCR assays to detect and quantitate siRNAs in vivo. These assays can be used to measure siRNA delivery efficiency after injections and follow body distribution. These assays can also be used to understand the RNAi mechanism and pathways in vivo.