Nanotech 2008 Vol. 2
Nanotech 2008 Vol. 2
Nanotechnology 2008: Life Sciences, Medicine & Bio Materials - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2

Drug & Gene Delivery Systems Chapter 4

Real-time Detection and Quantitation of siRNAs in vivo

Authors: A. Cheng, Y. Liang, A. Vlassov, M. Li, Y. Wang, L. Wong, C. Chen, S. Magdaleno

Affilation: Ambion Inc., An Applied Biosytems Business, United States

Pages: 427 - 429

Keywords: RNAi, in vivo, siRNAs, detection

Abstract:
We have developed TaqMan based RT-PCR assays to detect and quantitate siRNAs in vivo. These assays can be used to measure siRNA delivery efficiency after injections and follow body distribution. These assays can also be used to understand the RNAi mechanism and pathways in vivo.

Real-time Detection and Quantitation of siRNAs in vivo

ISBN: 978-1-4200-8504-4
Pages: 808
Hardcopy: $159.95