 | Nanotech 2008 Vol. 2
Nanotechnology 2008: Life Sciences, Medicine & Bio Materials - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Chapter 3: Biomarkers, Nano Particles & Materials |
Subcellular fractionation in a fluidic microsystem | |
| Authors: | A. Paulus, A. Posch, M. Hausmann, G. Blankenstein, T. Duong, J. Kentsch, B. Angres, D. Stoll, M. Stelzle |
| Affilation: | NMI Natural and Medical Sciences Institute, DE |
| Pages: | 287 - 288 |
| Keywords: | subcellular fractionation, biological nanoparticles, organelle, dielectrophoresis |
| Abstract: | The availability of an easy-to-use, reproducible method for subcellular fractionation would significantly promote qualitative and quantitative analysis of subcellular protein composition. A microfluidic system with integrated electrodes was designed and fabricated allowing for dielectrophoretic manipulation of biological nanoparticles. Fluorescently labelled mitochondria were successfully retrieved from a complex cell homogenate employing a dielectrophoretic band-bass electrode array. Dielectric properties of mitochondria were also investigated and biochemical analysis of fractions obtained was performed. |
| ISBN: | 978-1-4200-8504-4 |
| Pages: | 808 |
| Hardcopy: | $159.95 |
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