Nano Science and Technology Institute
Nanotech 2008 Vol. 2
Nanotech 2008 Vol. 2
Nanotechnology 2008: Life Sciences, Medicine & Bio Materials - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Chapter 3: Biomarkers, Nano Particles & Materials

Subcellular fractionation in a fluidic microsystem

Authors:A. Paulus, A. Posch, M. Hausmann, G. Blankenstein, T. Duong, J. Kentsch, B. Angres, D. Stoll, M. Stelzle
Affilation:NMI Natural and Medical Sciences Institute, DE
Pages:287 - 288
Keywords:subcellular fractionation, biological nanoparticles, organelle, dielectrophoresis
Abstract:The availability of an easy-to-use, reproducible method for subcellular fractionation would significantly promote qualitative and quantitative analysis of subcellular protein composition. A microfluidic system with integrated electrodes was designed and fabricated allowing for dielectrophoretic manipulation of biological nanoparticles. Fluorescently labelled mitochondria were successfully retrieved from a complex cell homogenate employing a dielectrophoretic band-bass electrode array. Dielectric properties of mitochondria were also investigated and biochemical analysis of fractions obtained was performed.
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