Nanotech 2008 Vol. 2
Nanotech 2008 Vol. 2
Nanotechnology 2008: Life Sciences, Medicine & Bio Materials - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2

Biomarkers, Nano Particles & Materials Chapter 3

Subcellular fractionation in a fluidic microsystem

Authors: A. Paulus, A. Posch, M. Hausmann, G. Blankenstein, T. Duong, J. Kentsch, B. Angres, D. Stoll, M. Stelzle

Affilation: NMI Natural and Medical Sciences Institute, Germany

Pages: 287 - 288

Keywords: subcellular fractionation, biological nanoparticles, organelle, dielectrophoresis

Abstract:
The availability of an easy-to-use, reproducible method for subcellular fractionation would significantly promote qualitative and quantitative analysis of subcellular protein composition. A microfluidic system with integrated electrodes was designed and fabricated allowing for dielectrophoretic manipulation of biological nanoparticles. Fluorescently labelled mitochondria were successfully retrieved from a complex cell homogenate employing a dielectrophoretic band-bass electrode array. Dielectric properties of mitochondria were also investigated and biochemical analysis of fractions obtained was performed.

Subcellular fractionation in a fluidic microsystem

ISBN: 978-1-4200-8504-4
Pages: 808
Hardcopy: $159.95