Authors: K-C Tsai, M-J Shieh, S-J Yang, W-L Lin, W-S Chen
Affilation: Institute of Biomedical Engineering, Taiwan
Pages: 26 - 29
Keywords: photodynamic medicine, 5-Aminolevulinic acid, chitosan, nanoparticle, ultrasound
Photodynamic medicine is a novel approach for cancer detection and treatment via different photosensitizers and suitable light source. The 5-Aminolevulinic acid (5-ALA) can be converted into protoporphyrin IX (PpIX) that can be utilized as a fluorescent probe for tumor detection. However, the fluorescent quantity of PpIX sometimes is low for detection. In this study, 5-ALA was encapsulated in chitosan nanoparticles, and then HeLa cancer cells uptake was enhanced by ultrasound to increase the fluorescent quantity of PpIX. We mixed chitosan with 5-ALA to prepare chitosan nanoparticles loaded with 5-ALA (CNA) by ionic gelation method. The size and zeta potential of CNA were measured by transmission electron microscope and zetasizer, respectively. The size of CNA was 102.4 nm and the zeta potential was 25.0 mV that was enough to prevent particles from aggregating. The loading efficiency of 5-ALA in CNA was up to 90%. Bioassy results of CNA for HeLa cell was done, and the result revealed that HeLa cell could uptake CNA and the amounts of CNA uptaken by cell could be enhanced greatly by ultrasound. According to this concept, we designed a novel photodynamic detection system to enhance the accuracy of diagnosis for early cervical cancer.