Nano Science and Technology Institute
Nanotech 2008 Vol. 1
Nanotech 2008 Vol. 1
Nanotechnology 2008: Materials, Fabrication, Particles, and Characterization - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 3: Nano Surfaces & Interfaces

Au Nanoparticle Coupled- Surface Plasmon Resonance Immuno-sensor for Sensitivity Enhancement

Authors:H-S. Kim, J-H. Kim, S-H. Ko, Y-J. Cho, J-H Kim
Affilation:Ajou University, KR
Pages:483 - 486
Keywords:SPR, Au nanoparticle, sensor, label-free
Abstract:In order to overcome the sensitivity limitation of SPR, nanoparticle-coupled SPR biosensors have explored since nanoparticles may significantly enhance the sensitivity by 1-2 orders of magnitude. Although the nanoparticle conjugated method contributes to the enhancement of the sensitivity, it does not take an advantage of SPR from the viewpoint of label-free detection. Herein we demonstrate the sensitivity enhancement with Au nanoparticles coupled- SPR immuno-sensor chip on which the specific size and surface density of the particles controlled as a label-free detection system. Au nanopariticles were synthesized and selected with a specific size. Surface density of immobilized 30 nm Au nanoparticles on bare Au film was estimated as 1x109 ea/cm2 using atomic force microscopy. With systematic control of the size of the Au nanoparticle and thickness of the bare Au film, it was found that 30 nm Au particles on 50 nm thick Au film demonstrated the largest resonance angle shift for surface reaction on the chip. This enhancement may result from both the effective surface area increase and the amplification of the resonance resonance effect. For human immunoglobulin G antibody-antigen analysis, this Au nanoparticle immobilized Au substrate shows highest SPR sensitivity comparing to the conventional bare Au film chip.
Au Nanoparticle Coupled- Surface Plasmon Resonance Immuno-sensor for Sensitivity EnhancementView PDF of paper
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