Nano Science and Technology Institute
Nanotech 2007 Vol. 3
Nanotech 2007 Vol. 3
Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 3
Chapter 4: Micro and Nano Fluidics

On-site Screening of Avian Influenza Virus (AIV) by Polystyrene Microfluidic Chip Immunoassay

Authors:Y. Zhao, M. Zou, Q. Xue and P. Zhou
Affilation:The Chinese Academy of Inspection and Quarantine, CN
Pages:399 - 402
Keywords:polystyrene microfluidic chip, micro-channels, AIV
Abstract:AIV Ag of A type with viral nucleoprotein (NP), which covers all AIV subtypes, including high-pathogenic AIV was determined by polystyrene microfluidic chip ELISA method. In micro-channels of chip instead of conventional 96-well microtiter plates, a double-antibody sandwich ELISA (DAS-ELISA) was developed, in which rabbit Ab, mouse nuclear protein (NP) mAb and horseradish peroxidase-conjugated goat anti-mouse immunoglobin were used as coating, primary and secondary antibodies, respectively. Recombine Ag of AIV was prepared as positive control to ensure the on-site test safety. With TMB as substrate, AIV Ag could distinctly be judged by comparing colors of sample detection windows with that of the negative control in micro-channels, a preliminary result was obtained in accordance with conventional ELISA while total analytical time were within 1 h. The on-chip DAS-ELISA was well applicable to rapid and real-time screening of AIV. The sensitivity could be further improved by optimization experiments.
On-site Screening of Avian Influenza Virus (AIV) by Polystyrene Microfluidic Chip ImmunoassayView PDF of paper
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