Authors: J-H Kim, S-W Choi, H-S Kim and Y-J Cho
Affilation: Ajou University, Korea
Pages: 124 - 127
Keywords: SPR, colloidal Au particle, chemical sensor
In order to overcome the sensitivity limitation of SPR, nanoparticle-coupled SPR biosensors have explored with tremendous interests since nanoparticles may significantly enhance the sensitivity by 1-2 orders of magnitude. M. J. Natan et al. reported that a conjugate between an antibody and Au colloidal particle produced a greater than 20-fold increase in plasmon angle shift over than observed in an assay using the unlabeled antibody. Though this colloid conjugated method is overcome the sensitivity, it does not take of advantage of SPR from the viewpoint of label-free detection. Herein we investigated the sensitivity enhancement in SPR chemical sensor coupling colloidal Au nanoparticles which have the specific size and surface density on sensor chips as label-free detection system.