Nano Science and Technology Institute
Nanotech 2007 Vol. 2
Nanotech 2007 Vol. 2
Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Chapter 6: Biosensors

Encapsulation of Streptavidin labeled with Alexa Fluor 750 into Erythrocytes

Authors:M. Dweik and S.A. Grant
Affilation:University of Missouri-Columbia, US
Pages:535 - 537
Keywords:red blood cells, encapsulation NIR dye
Abstract:Erythrocytes, Red Blood Cells (RBCs), have been utilized as biocompatible bioreactors and drug delivery vehicles. RBCs are also used as biological carriers for drugs, enzymes, and peptides. Hypo-osmotic dialysis technique is the most commonly employed to achieve drug encapsulation in RBCs with an appropriate yield. However, the use of RBCs as carriers for fluorescence labeled sensors has not been exploited. We have been investigating the feasibility of utilizing RBCs as optical carriers for biosensors. The first step is to ensure proper encapsulation and signal capture of the fluorescent probes. Streptavidin labeled with Alexa Fluor 750 (SA-AF 750) was utilized as the probe which was then loaded into the RBCs via the hypo-osmotic dialysis technique. The loaded cells were then scanned by using a spectrometer. The excitation scan was set at 750nm. The results showed a peak signal at 775 nm which was an indication of presence of AF 750 in the RBC’s. This model could be used in future work to design in vivo optical sensors and in vivo imaging.
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