Nano Science and Technology Institute
Nanotech 2007 Vol. 2
Nanotech 2007 Vol. 2
Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
 
Chapter 6: Biosensors
 

Electronic DNA detection on semiconductor surfaces

Authors:Y. Han, E. Tan, D. Candebat, K. Bandyopadhyay and A. Niemz
Affilation:Keck Graduation Institute, US
Pages:489 - 492
Keywords:SiO2/Si, EOS, impedance, DNA, EXPAR, gold nanoparticl
Abstract:Electronic biosensors offer a viable alternative to optical biosensors for the rapid, specific, and sensitive detection of DNA. We are utilizing an electrolyte-oxide-semiconductor (EOS) biosensor system on a SiO2/Si surface for impedimetric detection of DNA. Detection in this system is based on the intrinsic negative charge of DNA. Hybridization of target DNA to the probe-functionalized semiconductor electrode causes a change in the surface charge density, which results in a measurable shift of the impedance response. To covalently bind probe DNA sequences to the biosensor surface, we have activated and functionalized the SiO2/Si surface with aminosilanes, followed by crosslinking of amine-functionalized oligonucleotides to this surface displaying primary amine groups. To increase the sensitivity of our system, we are coupling a novel exponential isothermal DNA amplification reaction (EXPAR) with impedimetric DNA detection. We are further investigating the effect of co-immobilizing DNA functionalized Au nanospheres to the surface, using a sandwich assay format. Experimental results are compared to numerical simulations of different equivalent circuit models to assess the influence of main experimental parameters.
Electronic DNA detection on semiconductor surfacesView PDF of paper
ISBN:1-4200-6183-6
Pages:838
Hardcopy:$139.95
 
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