Authors: P.Y. Chen and K.C. Ho
Affilation: National Taiwan University, Taiwan
Pages: 477 - 480
Keywords: molecular imprinting, uric acid biosensor
In this work, the major contribution is fabricating a uric acid (UA) biosensor based on molecular imprinted technique using a novel conducting polymer. Calibration curves were depicted according to the amperometric detection of steady current in different concentrations of UA (from 0 to 1.125 mM). The sensitivities of molecularly imprinted polymer (MIP) and non-molecularly imprinted polymer (NMIP) electrodes were calculated to 22.59 and 9.67 μA mM-1cm-2, respectively. Imprinting efficiency, defined as the ratio of sensitivity of MIP to that of NMIP electrode, was 2.3 in this work. The limit of detection (LOD) for the MIP-ITO/Poly(PD-BCD)UA electrode was calculated to be 3×10-4 mM on the basis of signal to noise ratio (S/N) equaling to 3. It was found that the MIP sensor showed relatively good sensitivity, large linear range, and high imprinting efficiency with a low LOD for sensing UA. The MIP-ITO/Poly(PD-BCD)UA was also tested for ascorbic acid (AA), a major interferent, under its normal concentration range in human plasma. The current response of 0.04 mM AA is less than 7 % of the current response of 0.4 mM UA. This shows that AA has a low interference for the MIP-ITO/Poly(PD-BCD)UA electrode in sensing UA.