Authors: S. Pathak, E. Cao, M. Davidson, S. Jin and G. Silva
Affilation: UCSD, United States
Pages: 713 - 716
Keywords: quantum dots, neurosciences, labeling, GFAP, beta-tubulin
We have developed labeling methods using commercially available quantum dots specific to neurons and glia (Pathak S, in press) and have used these tools to label GFAP intermediate filament cellular bridges as well as integrin receptors on live Astrocytes. Since quantum dots are bright and provide detail into cellular interactions, previously unidentifiable characteristics, such as GFAP bridges, are now visible. In addition, we have developed computational analysis tools that perform automated tracking of quantum dot-target ligand dynamics in order to allow us to extract quantitative information about the spatial and temporal dynamics of individual labeled cell proteins.