Authors: H. Tsutsumi, M. Yamada and M. Yasui
Affilation: Yamaguchi University, Japan
Pages: 428 - 431
Keywords: CdSe, terpolymer, antibody, water-soluble
Fluorescent labeling reagents are used as diagnosis and research reagents that label specific protein, antigen, antibody, and DNA. However, the chromophoric groups are not stable under long-time Ultra Violet (UV) excitation measurement condition. Nanometer-sized semiconductor particles (for example, CdS or CdSe nanometer-sized particles) provide high intensity fluorescence with durability under long-time UV irradiation. However, the CdSe particles are usually insoluble to water. We prepared terpolymers which consist of water-soluble and hydrophobic parts, and functional group for antibody binding site. Water-soluble part in the polymers is composed of N-isopropylacrylamide. Hydrophobic part for anchoring to CdSe nanoparticles coated with hydrophobic stabilizing reagents and oleic acid is prepared by copolymerization of oleic acid (Ole) as a monomer. The terpolymers were prepared by radical polymerization of Nipam, Ole, and acrylic acid and their structures were confirmed by IR and NMR spectroscopic techniques. Coupling of the terpolymer-modified CdSe nanoparticles and monoclonal antibody (anit-tubulin antibody was used.) was performed by using water-soluble carbodiimide, EDC. Cleary stained tubulin in the HeLa cell was observed by using the antibody modified CdSe nanoparticles.