 | Nanotech 2006 Vol. 2
Technical Proceedings of the 2006 NSTI Nanotechnology Conference and Trade Show, Volume 2
Chapter 6: Medical Imaging through Nanotechnology |
CdSe Nanoparticles Modified with Water-soluble Terpolymers and their Application to Biomarkers | |
| Authors: | H. Tsutsumi, M. Yamada and M. Yasui |
| Affilation: | Yamaguchi University, JP |
| Pages: | 428 - 431 |
| Keywords: | CdSe, terpolymer, antibody, water-soluble |
| Abstract: | Fluorescent labeling reagents are used as diagnosis and research reagents that label specific protein, antigen, antibody, and DNA. However, the chromophoric groups are not stable under long-time Ultra Violet (UV) excitation measurement condition. Nanometer-sized semiconductor particles (for example, CdS or CdSe nanometer-sized particles) provide high intensity fluorescence with durability under long-time UV irradiation. However, the CdSe particles are usually insoluble to water. We prepared terpolymers which consist of water-soluble and hydrophobic parts, and functional group for antibody binding site. Water-soluble part in the polymers is composed of N-isopropylacrylamide. Hydrophobic part for anchoring to CdSe nanoparticles coated with hydrophobic stabilizing reagents and oleic acid is prepared by copolymerization of oleic acid (Ole) as a monomer. The terpolymers were prepared by radical polymerization of Nipam, Ole, and acrylic acid and their structures were confirmed by IR and NMR spectroscopic techniques. Coupling of the terpolymer-modified CdSe nanoparticles and monoclonal antibody (anit-tubulin antibody was used.) was performed by using water-soluble carbodiimide, EDC. Cleary stained tubulin in the HeLa cell was observed by using the antibody modified CdSe nanoparticles. |
 | View PDF of paper |
| ISBN: | 0-9767985-7-3 |
| Pages: | 893 |
| Hardcopy: | $119.95 |
| Order: | Mail/Fax Form |
| Special: | 3 CD Set — 15% off with Free Shipping |
| Up |
© 2013 Nano Science and Technology Institute. All Rights Reserved.
Terms of Use | Privacy Policy | Contact Us | Site Map
Terms of Use | Privacy Policy | Contact Us | Site Map