Nanotech 2006 Vol. 2
Nanotech 2006 Vol. 2
Technical Proceedings of the 2006 NSTI Nanotechnology Conference and Trade Show, Volume 2

Bio Sensors and Diagnostics Chapter 3

Preservation of DNA and Protein Biofunctionality for Bio-MEMS & NEMS Fabrication

Authors: D. Trau, J. Jie and N.J. Sucher

Affilation: National University of Singapore (NUS), Singapore

Pages: 294 - 296

Keywords: microfabrication, DNA, protein, biomicrodevices

Microfabrication processes especially in silicon are not compatible with biomolecules. For example, silicon processing is commonly performed at high temperatures and makes use of aggressive chemicals. Under these conditions biological materials are denaturated or decomposed. Currently biological materials can only be combined with microdevices after the last microfabrication production step.<br>Here we report the integration of DNA and protein into the microfabrication process using a gold passivation mask preserving their biofunctionality. This technology enables the integration of DNA and potentially other biomolecules into mass scalable microfabrication processes for biomedical devices, biochips, and biosensors.The gold film served as a biocompatible and stable passivation mask in the subsequent microfabrication processes. Biomolecules were patterned and aligned with microstructures formed by wet etching and microelectrodes created by a metal deposition/lift-off process. After microfabrication the gold passivation mask was removed under mild conditions to uncover the DNA or proteins. A prototype device was fabricated and biofunctionality of DNA was almost completely preserver; demonstration the full integration of DNA into silicon microfabrication for the first time.

Preservation of DNA and Protein Biofunctionality for Bio-MEMS & NEMS Fabrication

ISBN: 0-9767985-7-3
Pages: 893
Hardcopy: $119.95