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 | Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 8: Bio Micro Sensors |
| | New Light Induced Molecular Switch allows Sterically Oriented Micrometer Sized Immobilization of Biomolecules | | Authors: | T. Snabe, M.T. Neves-Petersen, P. Fojan, S. Klitgaard and S.B. Petersen | | Affilation: | Aalborg University, DK | | Pages: | 398 - 401 | | Keywords: | biosensor, UV, protein, immobilization, nanoarray | | Abstract: | Fundamental knowledge on protein structures and the effect of ultraviolet light on these structures has paved the way for the development of a unique light-based immobilization technology that allows oriented protein immobilization onto micrometer sized spots. The methodology is considered to be a strong alternative to the conventional procedures which often include the use of harsh conditions such as strong chemicals and elevated temperatures. The technology behind this immobilization technique - here termed 'light assisted immobilization' - is based on the fact that disulphide bridges that are naturally present within the protein structure can be broken as a result of UV-illumination. The free thiol groups (-SH) created upon disruption of a disulphide bridge are very reactive and can be used as linkers for covalent attachment to a surface. The surface can for example be gold or thiol-derivatized silicon, making this technology extremely useful for a large range of application areas, including biosensors. | | ISBN: | 0-9767985-0-6 |
| Pages: | 844 |
| Hardcopy: | $165.00 |
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| Order: | Mail/Fax Form |
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