 | Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 8: Bio Micro Sensors |
New Light Induced Molecular Switch allows Sterically Oriented Micrometer Sized Immobilization of Biomolecules | |
| Authors: | T. Snabe, M.T. Neves-Petersen, P. Fojan, S. Klitgaard and S.B. Petersen |
| Affilation: | Aalborg University, DK |
| Pages: | 398 - 401 |
| Keywords: | biosensor, UV, protein, immobilization, nanoarray |
| Abstract: | Fundamental knowledge on protein structures and the effect of ultraviolet light on these structures has paved the way for the development of a unique light-based immobilization technology that allows oriented protein immobilization onto micrometer sized spots. The methodology is considered to be a strong alternative to the conventional procedures which often include the use of harsh conditions such as strong chemicals and elevated temperatures. The technology behind this immobilization technique - here termed 'light assisted immobilization' - is based on the fact that disulphide bridges that are naturally present within the protein structure can be broken as a result of UV-illumination. The free thiol groups (-SH) created upon disruption of a disulphide bridge are very reactive and can be used as linkers for covalent attachment to a surface. The surface can for example be gold or thiol-derivatized silicon, making this technology extremely useful for a large range of application areas, including biosensors. |
 | View PDF of paper |
| ISBN: | 0-9767985-0-6 |
| Pages: | 844 |
| Hardcopy: | $109.95 |
| Order: | Mail/Fax Form |
| Special: | 3 CD Set — 15% off with Free Shipping |
| Up |
© 2013 Nano Science and Technology Institute. All Rights Reserved.
Terms of Use | Privacy Policy | Contact Us | Site Map
Terms of Use | Privacy Policy | Contact Us | Site Map