Nano Science and Technology Institute
Nanotech 2005 Vol. 1
Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 8: Bio Micro Sensors

Surface Characterization of a Direct, Label-Free Piezoelectric Immunosensor Platform

Authors:A. Papadopoulou-Bouraoui, M. Lejeune, J. Barrero-Moreno, A. Valsesia, M. Manso, D. Gilliland, G. Ceccone, and F. Rossi
Affilation:European Commission - JRC, IT
Pages:394 - 397
Keywords:quartz crystal microbalance, QCM, immunosensor, ellipsometry, AFM, TOF-SIMS, XPS, gold nanoparticles
Abstract:During this study the QCM (Quartz Crystal Microbalance) crystal surface was functionalized in order to provide active amino groups, and serve as an active platform for antibody immobilization and the development of direct, label-free immunosensors. This was achieved through the formation of a Self Assembled Monolayer (SAM) using 4-aminothiophenol (4-ATPh) and through plasma deposition of allylamine polymer film (ALL). Two different antibody immobilization routes were investigated during this study. The first involved the use of glutaraldehyde as a linker, followed by protein A (PA) for optimal antibody orientation and consecutively highly sensitive antibody-antigen interaction. The second route involved the use manometer-sized gold (20 nm) particles (GNP) in combination with PA. Complete characterization of each activation step was performed by QCM, Ellipsometry, Atomic Force Microscopy, Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) and X-Ray Photoelectron Spectroscopy (XPS). The combination of surface analytical, optical and mass balance techniques is confirming the effectiveness of these immunosensor fabrication strategies.
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