Nanotech 2005 Vol. 1
Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1

Bio Micro Sensors Chapter 8

Label Free Colorimetric Detection of Single Nucleotide Polymorphisms in Pcr Amplified Genomic DNA

Authors: H.X. Li and L.J. Rothberg

Affilation: University of Rochester, United States

Pages: 472 - 473

Keywords: PCR, DNA, gold nanoparticle, colorimetric detection, Au nanoparticles

Abstract:
Analysis of DNA is increasingly important in clinical diagnosis, pathology and genetics1, 2. Single nucleotide polymorphisms (SNPs) in genomic DNA are known to be responsible for a number of hereditary conditions3 and cancers4. Nearly all assays for DNA are based on the polymerase chain reaction (PCR), a rapid, inexpensive and simple means of amplifying specific sequence segments from as little as a single copy of DNA to easily detected quantities5, 6. Post processing of PCR amplified samples can, however, be expensive and time-consuming7. Here we demonstarte a label free colorimetric assay for SNPs in PCR amplified genomic DNA within ten minutes without need of time-consuming labeling, surface functionalization processes and additional detection instrumentation8. This assay is based on our new observation that single stranded DNA (ss-DNA) adsorbs on gold nanoparticles (Au-np) with a rate that depends on sequence length and temperature, and the adsorption can prevent the Au-np against salt-induced aggregation.

Label Free Colorimetric Detection of Single Nucleotide Polymorphisms in Pcr Amplified Genomic DNA

ISBN: 0-9767985-0-6
Pages: 844
Hardcopy: $109.95