 | Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 8: Bio Micro Sensors |
Label Free Colorimetric Detection of Single Nucleotide Polymorphisms in Pcr Amplified Genomic DNA | |
| Authors: | H.X. Li and L.J. Rothberg |
| Affilation: | University of Rochester, US |
| Pages: | 472 - 473 |
| Keywords: | PCR, DNA, gold nanoparticle, colorimetric detection, Au nanoparticles |
| Abstract: | Analysis of DNA is increasingly important in clinical diagnosis, pathology and genetics1, 2. Single nucleotide polymorphisms (SNPs) in genomic DNA are known to be responsible for a number of hereditary conditions3 and cancers4. Nearly all assays for DNA are based on the polymerase chain reaction (PCR), a rapid, inexpensive and simple means of amplifying specific sequence segments from as little as a single copy of DNA to easily detected quantities5, 6. Post processing of PCR amplified samples can, however, be expensive and time-consuming7. Here we demonstarte a label free colorimetric assay for SNPs in PCR amplified genomic DNA within ten minutes without need of time-consuming labeling, surface functionalization processes and additional detection instrumentation8. This assay is based on our new observation that single stranded DNA (ss-DNA) adsorbs on gold nanoparticles (Au-np) with a rate that depends on sequence length and temperature, and the adsorption can prevent the Au-np against salt-induced aggregation. |
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| ISBN: | 0-9767985-0-6 |
| Pages: | 844 |
| Hardcopy: | $109.95 |
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