Nano Science and Technology Institute
Nanotech 2005 Vol. 1
Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 8: Bio Micro Sensors

Micropatterned Laminin-1 Matrices

Authors:N. Sgarbi, D. Pisignano, F. Di Benedetto, A. Aloisi, G. Nicolardi, R. Cingolani and R. Rinaldi
Affilation:Leece University, IT
Pages:449 - 451
Keywords:laminin, micropatterning, self-assembly, cell
Abstract:We have obtained self-assembled laminin-1 networks on glass, realized by one-step microcontact printing lithography, without any preliminary functionalization of the substrates. We investigated the realized patterns by indirect immunofluorescence and atomic force microscopy (AFM), in order to assess their uniformity, the well-retained antigenicity of the proteins, and their supramolecular structural organization (Fig.1). We have demonstrated both the characteristic protein self-assembling in polygonal networks with well-defined sub-100 nm quaternary structures of laminin and that this result is strongly dependent on the LN-1 initial concentration. Currently, we are studying the interaction of these patterned substrates with cultured cells.
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