Authors: X.Y. Peng, P.C.H. Li, H-Z Yu, A.M. Parameswaran, S. Beardmore, W.L. Chou
Affilation: Simon Fraser University, Canada
Pages: 332 - 335
Keywords: microarray, microfluidic, DNA hybridization
DNA microarrays are usually constructed using photolithographic synthesis of oligonucleotide probes or by spotting of pre-synthesized probes. These are expensive methods, and only one sample can be applied on a microarray chip at one time. So we developed a new method that multiple samples (96) can be tested on a single microarray chip immobilzed with multiple probes (96). This method uses a microfluidic assembly, which includes 2 channel plates and 1 common chip in the form of the 3.5-inch compact dick (CD). When channel plate 1 is sealed against the common chip, liquid flow inside radial microchannels allows the immobilization of a line microarray of DNA probes. When channel plate 2 is sealed against the common chip, liquid flow inside spiral microchannels allows the formation of a spot microarray with DNA samples after hybridization. Small volume (1mL) and low concentration (1nM or lower) of multiple samples were achieved. Liquid flow was driven by centrifugal force obtained by spinning the CD. In addition, the probe immobilization time and sample hybridization time are much shortened. The hybridization time was three minutes. The detection limit was 1 nM if the sample volume was 1ml, or 1 fmol.