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Nanotech 2005 Vol. 1
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Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
 
Chapter 7: DNA, Protein, Cells and Tissue Arrays
 

Cationic Polythiophene for Label-Free, Colorimetric and Fluorometric Detection of DNA

Authors:H.-A. Ho and M. Leclerc
Affilation:Université Laval, CA
Pages:371 - 373
Keywords:conducting polymer, DNA detection, electrostatic interaction
Abstract:In order to respond to new demands and needs in modern medical diagnostics and biomedical research applications, a lot of new DNA detection approaches have been reported. However, most of these methods have the disadvantage of requiring the chemical coupling of a photoactive or electroactive tagging agent onto the target or the probe prior to detection. Some oligonucleotide-functionalized conjugated polymers have enabled the transduction of hybridization events, without labeling of the DNA target. Here we describe the use of a water-soluble, cationic polythiophene that can specifically transduce the binding of an appropriate oligonucleotide to its target into a clear optical (colorimetric or fluorometric) signal. This simple, rapid, sensitive and selective methodology does not require any chemical modification on the probes or targets.
ISBN:0-9767985-0-6
Pages:844
Hardcopy:$165.00
 
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