 | Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 4: Bio Nano Particles |
Quantum Dot-Mediated Separation-Free Assay for Point Mutation Detection | |
| Authors: | H.C. Yeh, Y.P. Ho and T.H. Wang |
| Affilation: | Johns Hopkins University, US |
| Pages: | 198 - 201 |
| Keywords: | quantum dots, single nucleotide polymorphisms, coincidence detection |
| Abstract: | We describe a novel semiconductor quantum dot (QD)-based biosensing assay for single nucleotide polymorphism (SNP) detection. By integrating a QD-mediated dual-color fluorescence coincidence detection method with oligonucleotide ligation assay (OLA), we have achieved rapid SNP detection in a homogenous separation-free format. Detection of target signal is carried out on a single quantum dot basis using ultrasensitive confocal fluorescence spectroscopy. Comparing with other separation-free SNP detection schemes such as molecular beacon and microparticle-based flow cytometry, our assay shows advantages in much enhanced sensitivity and speed. Moreover, we have demonstrated that this QD-based assay allowed SNP detection with nearly infinite specificity. Our developed QD-based SNP assay requires no need for immobilization and wash after duplex denaturation, no need for complicated probe design, and no need for temperature control during measurement, rendering this a simple, fast, cost effective, and potentially an on-line monitoring assay for point mutation detection. |
 | View PDF of paper |
| ISBN: | 0-9767985-0-6 |
| Pages: | 844 |
| Hardcopy: | $109.95 |
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