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 | Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 3: Drug Delivery |
| | Nano-Structured Vaccine Delivery | | Authors: | Y-W Yang and C-A Wu | | Affilation: | National Taiwan University, TW | | Pages: | 140 - 143 | | Keywords: | vaccine, adjuvant, delivery | | Abstract: | The nano-structured vaccine delivery systems, containing tomatine, n-octyl-b-D-glucopyranoside (OGP), phosphatidylethanolamine (PE), cholesterol, and ovalbumin, were examined in the present study by transmission electron microscopy. This adjuvant has been shown to elicit cytoxic T-lymphocyte (CTL) effect when co-immunized with the antigens. Transmission electron microscopy was carried out to examine the nano-structures of this complex vaccine delivery system and its interaction with the dendritic cells, the most powerful antigen-presenting cells in vitro. To examine the nano-structures, tomatine adjuvant was separated by isopycnic ultracentrifugation. Examination by transmission electron microscopy revealed the heterogeneity characteristics of the adjuvant, containing several micro- and nano- structures with the major fraction consisting of cylindrical-shaped nano-crystals, approximately 80-160 nm in width and 2-4 mm in length (Fig. 1). Tomatine alone in 0.9% NaCl, on the other hand, was shown to be cylindrical crystals of hundreds of nanometers up to a few microns in length, along which n-octyl-b-glucopyranoside was speculated to serve as a seeding nano- templates for complexation with the antigens. Several other forms of micro- and nano-structures were observed, showing multiple dispersion features with the nano-gel characteristics. The presence of the nano-gel structures was speculated to correlate with the sustained immunopotentiation effect of tomatine adjuvant. | | ISBN: | 0-9767985-0-6 |
| Pages: | 844 |
| Hardcopy: | $165.00 |
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