 | Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 2: Medical Applications |
Conical Tomograph: A Method for the Study of Macromolecular Assemblies | |
| Authors: | L. Zampighi, N. Fain, S. Lanvazecchia, G.A. Zampighi |
| Affilation: | University of California, Los Angeles School of Medicine, US |
| Pages: | 59 - 62 |
| Keywords: | TEM, tomography, synapses, vesicles, proteins |
| Abstract: | We have developed conical tomography to study the structure of macromolecular assemblies and organelles in their native cellular environment. Unlike the other related types of tomography, conical tomography has a higher throughput and more importantly, produces reconstructions that are isotropic in the x-y plane with a resolution of 2-3 nm. We have been able to collect complete conical series from a variety of sources, including thin sections and freeze-fractured replicas of chemical synapses from the rat somato-sensory cortex as well as isolated synaptic vesicle anti-SV2 complexes. We used volumetric representations and density segmentation methods to analyze sections, revealing a number of highly detailed structures: tubes and cisterns were found in the presynaptic terminal, as well as protein cages formed of SV-2 rings, circumscribing the synaptic vesicles, and a number of docked vesicles and omega figures. While conical tomography is a general technique, theoretically applicable to any types of structure on the molecular scale, we are currently focused on the synaptic terminal and the distribution of the ~60 proteins associated with it. Subsequent analysis promises to provide information about it's structure/function, including clues as to it's role in Alzheimer's, Parkinson's, drug addiction, and other diseases. |
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| ISBN: | 0-9767985-0-6 |
| Pages: | 844 |
| Hardcopy: | $109.95 |
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