Nanotech 2005 Vol. 1
Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1

Medical Applications Chapter 2

Nanomagnetic Immunoassay for Rapid Detection of Escherichia coli

Authors: Y. Haik, A. Qablan and C-J Chen

Affilation: Center for Nanomagnetics and Biotechnology, United States

Pages: 81 - 84

Keywords: nanomagnetic, immunoassay, E.Coli

Abstract:
This study identifies a simple process to quickly conduct an analysis, under field conditions, for detection of pathogens. A magnetic sandwich immunoassay is developed for rapid isolation and detection of targeted pathogen. Escherichia coli BL21 Star (DE3) pLysS is used as a model in this study. The magnetic immunoassay takes advantage of the multiple epitopes that are found on the target. On one epitope a nanomagnetic particle is attached. On another epitope a glucose molecule is attached. The sandwich magnetic assay that contains the glucose label can be easily correlated to the concentration of the Escherichia coli BL21 Star (DE3) pLysS.

Nanomagnetic Immunoassay for Rapid Detection of Escherichia coli

ISBN: 0-9767985-0-6
Pages: 844
Hardcopy: $109.95