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 | Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 11: Microfluidics and Lab on Chip |
| | Development of Microfluidic Device for Heterogeneous Immunoassay on Polystyrene Platform with Electroosmotic Control | | Authors: | M. Pribyl, D. Snita and M. Marek | | Affilation: | Institute of Chemical Technology Prague, CZ | | Pages: | 672 - 675 | | Keywords: | heterogeneous immunoassay, electroosmosis, microchip, microdevice | | Abstract: | We have developed a microfluidic device for heterogeneous immunoassay on the polystyrene platform. The wire-imprinting method has been chosen for the construction of microchannel structure of the chip. Immunoassay based on the interaction of fluorescently labeled human IgG (FITC-IgG) and Protein A (antigen) is selected as experimental system. The sample and other solutions are manipulated only by the electroosmotic mechanism. We have measured the electroosmotic mobility coefficient in the microchannels equal to mu_eo = 2.1 10-8 m2V-1s-1. Mathematical model of the microchip of the same geometry based on the transport-reaction equations, the equation of electrostatics, the momentum balances and the slip boundary conditions was also constructed and analyzed. | | ISBN: | 0-9767985-0-6 |
| Pages: | 844 |
| Hardcopy: | $165.00 |
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