Nanotech 2004 Vol. 1
Nanotech 2004 Vol. 1
Technical Proceedings of the 2004 NSTI Nanotechnology Conference and Trade Show, Volume 1

Bio Nano Computational Methods and Applications Chapter 5

Resolved Particle Simulations of Microsphere/Cell-based Bioanalytic Systems

Authors: K. Pant, B.F. Romanowicz and S. Sundaram

Affilation: CFD Research Corporation, United States

Pages: 184 - 187

Keywords: microsphere, cell, assay, design, resolved particle simulations

Abstract:
Microsphere and cell-based assays are emerging as the platform of choice for rapid quantitation of biomolecular interactions in bioagent detection, clinical diagnosis, drug therapy, etc. [1,2] Traditional assay designs have been driven by expensive trial-and-error experimentation. The availability of high-fidelity simulation tools for these complex microsystems can help accelerate the design cycle significantly. However, current models rely on a point particle approximation and are incapable of describing large particle dynamics or biochemical coupling adequately. In a novel development, we have successfully developed computational models for finite-sized cells/bead assay design within a multiphysics biomicrofluidic simulation software suite, CFD-ACE+. The developed models fully integrate large bead dynamics, convective-diffusive analyte transport and biomolecular binding on bead surface. In this paper, we present the modeling framework, along with several validation and demonstration case studies.

Resolved Particle Simulations of Microsphere/Cell-based Bioanalytic Systems

ISBN: 0-9728422-7-6
Pages: 521
Hardcopy: $79.95