Sample-Shunting Based PCR Microfluidic Device

	Nanotech 2004 Vol. 1 Technical Proceedings of the 2004 NSTI Nanotechnology Conference and Trade Show, Volume 1 Chapter 2: Bio Nano Analysis and Characterization
	Sample-Shunting Based PCR Microfluidic Device
Authors:	P-A Auroux, P.J.R. Day and A. Manz
Affilation:	Imperial College, UK
Pages:	67 - 69
Keywords:	PCR, DNA, microfluidics, chip
Abstract:	A new method to perform on-chip DNA amplification is presented in this abstract: we shunt the sample back and forth over temperature-controlled zones. Compared to existing systems, this approach offers, in addition to a greater flexibility in terms of cycling programme, an incomparable ease of parallelisation. We report in this paper the first results obtained when using a sample-shunting approach to achieve on-chip PCR. An unoptimised chip displays an efficiency of 25% and is six times faster than a conventional thermo-cycler. To the extent of our knowledge no comparable results have been previously published.
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ISBN:	0-9728422-7-6
Pages:	521
Hardcopy:	$79.95

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