Nano Science and Technology Institute
Nanotech 2004 Vol. 1
Nanotech 2004 Vol. 1
Technical Proceedings of the 2004 NSTI Nanotechnology Conference and Trade Show, Volume 1
 
Chapter 2: Bio Nano Analysis and Characterization
 

Microfabricated Fluorescence-Activated Cell Sorter with Hydrodynamic Flow Manipulation

Authors:H. Bang, C. Chung, J.K. Kim, S. Kim, S. Chung, D-C Han and J.K. Chang
Affilation:School of Mechanical & Aerospace Eng., Seoul National University, KR
Pages:50 - 54
Keywords:micro-FACS, Lab-on-a-chip, hydrodynamic flow manipulation, plastic micromachining, PDMS
Abstract:Presented is a novel flow manipulation and particle detection method for the microfabricated fluorescence-activated cell sorter (FACS). With hydrodynamic flow manipulation which includes passive flow channeling and hydrodynamic actuation with nozzle flow, we have developed a fast and robust method for utilizing the sorting function. These techniques are directly related to enabling the high throughput screening of the FACS machine. Moreover by detecting with synchronized imaging, we have also developed an on-line calibration technique for the accurate timing between detection and actuation. And this means that our system is very flexible that it can be tuned to any assay conditions with various flow speeds, particle densities and buffer or sample viscosities by real time calibration.
Microfabricated Fluorescence-Activated Cell Sorter with Hydrodynamic Flow ManipulationView PDF of paper
ISBN:0-9728422-7-6
Pages:521
Hardcopy:$79.95
 
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