Bio Nano Analysis and Characterization

Overview of Overlapping Bio and Nano Technologies

Iyer S., LANL, US

“What is the connection between nanoscience and biology?” “What are biologists doing at Nanotech 2004 and why do you have so many “bio” related sessions?” “So biologists are also jumping on the nanoscience bandwagon.” These [...]

Detection of DNA Hybridyzation with Impedance Amplifying Labels

Ma K-S, Madou M., Zhou H., Fischetti A., Zoval J., Madou M., University of California Irvine, US

Traditionally used nucleic acid analysis detection platforms rely on fluorescent or radioactive labels. Optical techniques require large and expensive equipment such as automated colorimetric and fluorimetric instruments that greatly increase the cost of the whole [...]

Functional Nanoarrays for Protein Biomarker Profiling

Lynch M., Mosher C., Huff J., Nettikadan S., Xu J., Henderson E., BioForce Nanosciences, Inc., US

The success of microarrays for analyzing nucleic acid and, more recently, protein-protein interactions illustrates the advantages of bioassay miniaturization. However, the current level of miniaturization of useful bioanalytical tools still creates limits in terms of [...]

An Chip-based-Instant Protein Micro Array Formation and Detection System

Tseng F-G, Ho C.E., Chen M.H., Chen Y.F., Hung K.Y., Su C-J, Chen M.H., Chen Y.F., Huang H.M., Chieng C-C, National Tsing Hua University, TW

This paper proposes a novel protein micro arrayer employing 3-in-1 chip system, instantly delivering hundreds of biosamples in parallel for disease diagnosis. The 3-in-1chip system included: micro filling chip, micro stamper array and micro bio-reaction [...]

High-Resolution Electrochemical In Situ Printing

Egeland R.D., Southern E.M., Oxamer & Oxford Gene Technology, UK

This work demonstrates a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass [...]

Microfabricated Fluorescence-Activated Cell Sorter with Hydrodynamic Flow Manipulation

Bang H., Chung C., Chung S., Kim J.K., Kim S., Kim J.K., Kim S., Chung C., Chung S., Han D-C, Chang J.K., School of Mechanical & Aerospace Eng., Seoul National University, KR

Presented is a novel flow manipulation and particle detection method for the microfabricated fluorescence-activated cell sorter (FACS). With hydrodynamic flow manipulation which includes passive flow channeling and hydrodynamic actuation with nozzle flow, we have developed [...]

Development of a Slug-Flow PCR Chip with Minimum Heating Cycle Times

Hardt S., Dadic D., Doffing F., Drese K.S., Münchow G., Sörensen O., Institute of Microtechnology Mainz, DE

A microfluidic PCR device is presented capable of rapid temperature ramping and handling of sample volumes in the microliter and submicroliter range. The PCR chip comprises a straight micro channel in which a sample slug [...]

Composable System Simulation of Dispersion in Complex Electrophoretic Separation Microchips

Wang Y., Lin Q., Mukherjee T., Carnegie Mellon University, US

This paper presents a composable system simulation framework for electrophoretic separation microchips, using an analog hardware description language integrating analytical dispersion models that describe not only the behavior of individual components, but also the interactions [...]

Visualizing Individual Rhodopsin (a G Protein-Coupled Receptor) Molecules in Native Disk and Reconstituted Membranes via Atomic Force Microscopy

Choi E.J., Jin A.J., Niu S-L, Smith P.D., Litman B.J., National Institutes of Health, US

Individual rhodopsin molecules have been resolved with atomic force microscopy as both monomers and various oligomeric organizations that are sensitively dependent upon the physical state of membranes and environmental conditions. In intact native disk membranes, [...]

Sample-Shunting Based PCR Microfluidic Device

Auroux P-A, Day P.J.R., Manz A., Imperial College, UK

A new method to perform on-chip DNA amplification is presented in this abstract: we shunt the sample back and forth over temperature-controlled zones. Compared to existing systems, this approach offers, in addition to a greater [...]

Highly Efficient Electro-permeabilization of Mammalian Cells Using Micro-electroporation Chip

Shin Y.S., Kim J.K., Lim S.H., Lee Y., Lee Y.K., Cho K., Lee Y., Lee Y.K., Chung C., Chung S., Chung C., Chung S., Han D-C, Chang J.K., Seoul National University, KR

Electroporation, is widely used method that can be directly applied to the field of gene therapy. However, very little is known about the basic mechanisms of DNA transfer and cell response to the electric pulse. [...]

Effect of Substratum Morphology on Cell Behavior

Su W-T, Chu I-M, Yang J-Y, Lin C-D, National Nano Device Laboratories, TW

Contact guidance induced by the surface topography of the substrata influences the direction of cells with artificial material. Mouse bone marrow stromal fibroblast was examined on grooved substrata of varying dimension (0.3£gm~1.2£gm groove width, 0.5£gm [...]

An Injector Component Model for Complete Microfluidic Electrokinetic Separation Systems

Magargle R., Hoburg J.F., Mukherjee T., Carnegie Mellon University, US

This work presents a closed-form, numerically derived model of a key microfluidic electrokinetic separation system component, the cross injector. The model fits into a framework such that it can be combined with a network of [...]

Dielectrophoresis (DEP) of Cells and Microparticle in PDMS Microfluidic Channels

Kanagasabapathi T.T., Backhouse C.J., Kaler K.V.I.S., University of Calgary, CA

Dielectrophoresis (DEP) [1] The behavior of polarized, neutral particles to the non-uniform electric field has been successfully applied for the levitation and subsequent isolation of biological cells and other micron-sized inorganic particles [2, 3]. This [...]

Dry Microarrays: A Generalized Data Source for Genome and Proteome Analysis

Sampath G., The College of New Jersey, US

Microarray analysis methods can be applied to characteristic data obtained from biological sequences in matrix form (which could include data from experiments or bioassays as well). The rows correspond to a genome region (intron or [...]

Molecular Simulation of DNA Microarrays – an Application of 2D particle mesh Ewald algorithm

Watanabe T., Kawata M., Nagashima U., National Institute of Advanced Industrial and Science and Technology, JP

We have developed the two-dimensional particle mesh Ewald (2D-PME) algorithm for molecular dynamics (MD) simulation, and applied it to have knowledge of the behavior of DNA and its interaction on microarrays, which crucial in the [...]

Development and Characterization of a 2-D microchip-based Protein separation system

Feng J.J., Olazábal V, Olivares J.A., Sundaram S., Krishnamoorthy S., Iyer S., Los Alamos National Laboratory, US

Traditional methods in proteomics have relied on gel-based separation of proteins followed by directed cleavage of these proteins and mass spectrometry to measure the products. The emphasis on reliable, high-throughput analyses has led to the [...]

Amplification of Protein Adsorption on Micro/Nanostructures for Microarray Applications

Ivanova E.P., Alekseeva Y.V., Pham D.K., Filipponi L., Nicolau D.V., Swinburne University, AU

The fabrication and operation of biodevices require the accurate control of the concentration of the bioactive molecules on the surface, as well as the preservation of their bioactivity, in particular proteins that have a significant [...]

Evaluation of Electric Impedance Spectra for Single Bio-Cells with Microfluidic Devices using Combined FEMLAB/HSPICE Simulated Models

Senez V., Yamamoto T., Poussard B., Fukuba T., Capron J.M., Fujii T., LIMMS/CNRS-IIS - University of Tokyo, JP

This paper describes a simple method to predict the electrical impedance spectrum of single & cultured cells in micro-devices. It can be used for the rapid design of micro-sensors as well as for more fundamental [...]

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