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 | Nanotech 2003 Vol. 3
Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 3
Chapter 9: Biomimetic Membranes |
| | A Glucose Biosensor using Au Nanoparticle Modified Glucose Oxidase | | Authors: | X. Meng, X. Ren and F. Tang | | Affilation: | Chinese Academy of Sciences, CN | | Pages: | 404 - 407 | | Keywords: | biosensor, nanoparticles, glucose oxidase | | Abstract: | Biosensors have a wide field of potential applications in biotechnology, process control and medicine, mainly for accurate measurement of the analyte concentration of physiological fluid to diagnose some diseases. The development of enzyme-based biosensor technology for chemical, environmental and medical analysis has been very rapid. However, the requirement of an electron transfer mediator for most amperometric bioelectrodes has hindered the development and application of these devices. Efficient electrical communication between an immobilized enzyme and a solid electrode surface is necessary for monitored a current in an external circuit of amperometric enzyme biosensor. Such electron transfer requires electrical contact between the protein’s redox center and the electrode’ interface. In most of instances this is inhibited due to that the protein matrix separates the redox-site from the electrode surface and practically insulates the active center from electrical contact with electrode interfaces. Diffusional electron mediators as charge transporters and/or promoter molecules have been employed in order to improve the transfer of electrons between the active redox center of the enzyme and the electrode surface. At these modified electrodes rapid electron transfer can take place. |  | View paper | | ISBN: | 0-9728422-2-5 |
| Pages: | 560 |
| Hardcopy: | $125.00 |
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