Nano Science and Technology Institute
Nanotech 2003 Vol. 1
Nanotech 2003 Vol. 1
Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 1
 
Chapter 8: Nano Fluidics and Transport
 

Study of Sample Dispersion Mechanisms in an Electroosmotically Pumped Microchannel

Authors:B. Debusschere, H. Najm, A. Matta, O. Knio, R. Ghanem and O. Le Maitre
Affilation:Sandia National Laboratories, US
Pages:154 - 157
Keywords:dispersion, electroosmotic, stochastic, microchannel, protein labeling, zeta-potential
Abstract:In many microfluidic separation processes, dispersion of sample peaks is a significant problem limiting the detection resolution. In this work, we use simulations to study the dominant dispersion mechanisms during a protein labeling process in electroosmotic microchannel flow with a potassium phosphate buffer. The goal is to explain the dominant dispersion mechanisms and to provide a tool for predicting and minimizing dispersion in microchannel flows. The simulations are performed with a stochastic microchannel simulation code. We use a detailed representation of electroosmotic and pressure-driven channel flow by solving the coupled momentum, species transport, as well as electrostatic field equations. The chemistry model accounts for pH-dependent protein labeling reactions as well as detailed buffer electrochemistry in a mixed finite-rate/equilibrium formulation. The model also has the unique capability to account for stochastic processes, such as random zeta-potential variability. In our talk, we will specifically address two phenomena. The first is the electrokinetic and hydrodynamic dispersion, caused by the disturbance of the buffer solution as a sample moves through the channel. The second is the effect on dispersion of random zeta-potential variability, due to surface roughness or heterogeneous wall material properties.
Study of Sample Dispersion Mechanisms in an Electroosmotically Pumped MicrochannelView PDF of paper
ISBN:0-9728422-0-9
Pages:560
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