Nanotech 2003 Vol. 1
Nanotech 2003 Vol. 1
Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 1

Bio Micro Systems Chapter 7

Modeling AFM Induced Mechanical Deformation of Living Cells

Authors: R.E. Rudd, M. McElfresh, R. Balhorn, M. Allen and J. Belak

Affilation: Lawrence Livermore National Laboratory, United States

Pages: 138 - 141

Keywords: membrane, cell mechanics, atomic force microscopy, recognitition force microscopy

Abstract:
One challenge with using Atomic Force Microscopy (AFM) for recognition microscopy on living cells is the fact that the cell is not rigid, and as the force is applied to a receptor site, it is not just the receptor site that is affected. The whole cell deforms under the applied force, and the measured binding force is a convolution of the local, intrinsic binding force of the receptor site and the gross elastic response of the cell. We have developed a model of the elastic deformation of the cell in order to separate the two effects, based on a continuum level analysis of the elastic deformation, including the incompressible interior and the tension and curvature of the membrane. A novel feature of this formalism are the treatment of the Canham-Helfrich curvature strain for finite deformations under load. As a validation, the model has been compared with force-displacement curves coming from AFM nanoindentation experiments on both membrane vesicles and cells. The model allows site-specific mechanical properties to be deconvoluted from the gross cell deformation in recognition microscopy experiments. Eventually, it may be possible to use concurrent multiscale modeling to provide a model of the atomistic interactions at the receptor site too.


ISBN: 0-9728422-0-9
Pages: 560

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