Immunosensing Using Fabricated protein Microarrays

	Nanotech 2003 Vol. 1 Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 1 Chapter 5: Bio Arrays and Proteomics
	Immunosensing Using Fabricated protein Microarrays
Authors:	S.W. Howell, H.D. Inerowicz, L. Guirl, F. Regnier and R. Reifenberger
Affilation:	Purdue University, US
Pages:	74 - 77
Keywords:	protein microarray, bacteria, detection, SPM
Abstract:	Patterned microarrays of antibodies to specific bacteria were used to perform a series of bacterial immunoassays of E. Coli 0157:H7 and Renibacterium Salmoninarum (RS). Microarrays were fabricated using microcontact printing (MCP) and characterized using scanning probe microscopy (SPM). The high-resolution SPM imaging showed that bacteria had a higher binding affinity to antibody patterns than unfunctionalized regions of the substrates. Additional studies indicated a low binding affinity when bacteria were exposed to microarrays of non-specific antibodies. These studies demonstrate how protein microarrays could be developed into useful platforms for sensing microorganisms.
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ISBN:	0-9728422-0-9
Pages:	560
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