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 | Nanotech 2003 Vol. 1
Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 1
Chapter 5: Bio Arrays and Proteomics |
| | Immunosensing Using Fabricated protein Microarrays | | Authors: | S.W. Howell, H.D. Inerowicz, L. Guirl, F. Regnier and R. Reifenberger | | Affilation: | Purdue University, US | | Pages: | 74 - 77 | | Keywords: | protein microarray, bacteria, detection, SPM | | Abstract: | Patterned microarrays of antibodies to specific bacteria were used to perform a series of bacterial immunoassays of E. Coli 0157:H7 and Renibacterium Salmoninarum (RS). Microarrays were fabricated using microcontact printing (MCP) and characterized using scanning probe microscopy (SPM). The high-resolution SPM imaging showed that bacteria had a higher binding affinity to antibody patterns than unfunctionalized regions of the substrates. Additional studies indicated a low binding affinity when bacteria were exposed to microarrays of non-specific antibodies. These studies demonstrate how protein microarrays could be developed into useful platforms for sensing microorganisms. |  | View paper | | ISBN: | 0-9728422-0-9 |
| Pages: | 560 |
| Hardcopy: | $125.00 |
| Special: | 3 CD Set — 15% off with Free Shipping |
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