Nano Science and Technology Institute
Nanotech 2003 Vol. 1
Nanotech 2003 Vol. 1
Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 1
Chapter 5: Bio Arrays and Proteomics

Immunosensing Using Fabricated protein Microarrays

Authors:S.W. Howell, H.D. Inerowicz, L. Guirl, F. Regnier and R. Reifenberger
Affilation:Purdue University, US
Pages:74 - 77
Keywords:protein microarray, bacteria, detection, SPM
Abstract:Patterned microarrays of antibodies to specific bacteria were used to perform a series of bacterial immunoassays of E. Coli 0157:H7 and Renibacterium Salmoninarum (RS). Microarrays were fabricated using microcontact printing (MCP) and characterized using scanning probe microscopy (SPM). The high-resolution SPM imaging showed that bacteria had a higher binding affinity to antibody patterns than unfunctionalized regions of the substrates. Additional studies indicated a low binding affinity when bacteria were exposed to microarrays of non-specific antibodies. These studies demonstrate how protein microarrays could be developed into useful platforms for sensing microorganisms.
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